Meropenem (MERO) and Sulbactam (SUL) are used in combination in treatment of lower respiratory tract infections. Two simple, accurate, precise, economical and reproducible UV spectrophotometric methods have been developed for the estimation of Meropenem and Sulbactam in Pharmaceutical formulation. Method I- absorption ratio method (Q-analysis) using two wavelengths, 283 nm (isobestic point at which both the drugs exhibit absorbance) and 257.6 nm (λmax of Sulbactam) and Method II-First Derivative method using two wavelengths, 254 nm (ZCP of Meropenem) and 321 nm (ZCP of Sulbactam). Linearity for detector response was observed in the concentration range of 10-50 µg/ml & 5-25 µg/ml for Meropenem and Sulbactam respectively. The results of analysis have been validated statistically and by recovery studies the value of standard deviation was satisfactory and recovery studies ranging from 99.00-100.50% for Meropenem and 98.88-101.00% for Sulbactam in method-I and 99.72-100.50% for Meropenem and 98.00-100.45% for Sulbactam in method-II were indicative of the accuracy and precision of the proposed method. The proposed methods were successfully applied for the determination of Meropenem and Sulbactam in commercial pharmaceutical preparation. All two methods were validated statistically as per ICH guidelines.
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